Obesity has become a worldwide public health pandemic, associated with a rise in the prevalence of metabolic illnesses such as type 2 diabetes, cardiovascular disease, respiratory disease, musculoskeletal problems, liver disease, kidney disease, gallbladder disease, and cancer. Dietary phenolic compounds are considered as bioactive compounds that have effects in different chronic disorders related to oxidative stress, inflammation process, or aging. There is growing evidence that natural products, mostly plant-derived polyphenols, are important in the relationship between nutrients and health in humans. Recent in vitro and in vivo investigations indicate that polyphenolic extracts of Lippia citriodora (LC) reduce triglyceride buildup, increase fatty acid oxidation, and regulate glucose metabolism. The most well-known characteristic of obesity is the proliferation and differentiation of adipocytes from pre-adipocytes, which results in an increase in their number. We used an insulin-resistant hypertrophic 3T3-L1-adipocyte model in our study. MAPK signaling pathway, consisting of three groups of molecules-ERK 1/2, JNK, and p38, modulates the expression of adipogenic transcription factors. We investigated the effect of two different dosages of Lippia citriodora (250-400 g/ml) on cell survival and MAPK pathway in hypertrophic adipocytes cells. We determined the cell viability by WST-8 assay in hypertrophic 3T3-L1 adipocyte cells. Total/phosphorylated p38, JNK and ERK proteins in adipocyte cells were analyzed by using Colorimetric Cell-Based Assay. We found that treatment with LC suppressed the phosphorylated ERK1/2, phosphorylated p-38 and JNK. Anti- obesity mechanisms of LC could mediated by p38‐MAPK/JNK/ERK pathway in hypertrophic 3T3-L1 adipocyte cells.
Anahtar Kelimeler: Adipocyte cells, Hypertropy, 3T3-L1, Lippia citriodora, MAPK
|
