Obesity is a disease with a continuous increase in the frequency and it is known that the factors affecting this are age, gender, race, sociocultural level, existence of obese individuals in the family and eating habits. It has been postulated that dietary polyphenols could be used as therapeutic agents for the treatment of obesity. Different cell lines have been widely used as a model to study metabolic disturbances related to obesity. Despite the limitations of a cell model, the 3T3-L1 adipocytic cell line under glucolipotoxic conditions is a well-established cell model to study the effect of these phytochemicals and their metabolites on obesity-associated metabolic disturbances. Polyphenols are bioactive molecules that are present in plant-based foods. Dietary intake of plant polyphenols has been associated with several health outcomes related t oxidative stress, including cardiovascular diseases, hypertension, diabetes, mortality and some cancers. The potential anti-obesity activity and mechanisms of MetabolAid® has been investigated over the last few decades. MetabolAid® has been shown in several in vivo and in vitro experiments to lower body fat accumulation and adipocyte size. The purpose of our study was to determine the MAPK pathway family members JNK, p38 and ERK in hypertrophied 3T3-L1 adipocytes. We have found that MetabolAid® inhibited lipid accumulation and downregulated the MAPK signaling pathway in adipocytes. This study for the first time depicts the functional properties of MetabolAid® on MAPK patway in hypertrophied adipocytes. JNK-p38-ERK signaling which may pave the way for further research in therapeutics against the obesity.
Anahtar Kelimeler: 3T3-L1, adipocyte, MAPK, obesity, polyphenol
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